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目的探讨平喘汤对支气管哮喘大鼠的干预作用。方法将SD大鼠48只随机分为正常对照组、哮喘模型组、平喘汤低剂量组、平喘汤中剂量组、平喘汤高剂量组、阳性药物(博利康尼)对照组。采用卵蛋白致敏复制大鼠支气管哮喘模型,治疗组从实验开始第3周起,平喘汤高、中、低剂量组以2.6 mL/次、1.3 mL/次、0.7 mL/次剂量灌胃,阳性药物(博利康尼)对照组0.1 mL/次剂量灌胃,正常组及模型组均给予1.3 mL/次生理盐水灌胃,每天一次,连续4周。观察大鼠一般情况,大鼠肺组织IgE、TGFβ1及TNF-α的变化。结果哮喘组及各治疗组大鼠肺组织IgE、TGFβ1及TNF-α含量均高于正常组,差异显著(P<0.05);各治疗组大鼠肺组织IgE、TGFβ1及TNF-α含量均低于哮喘组,有显著性差异(P<0.05);与博利康尼组相比,平喘汤高剂量组大鼠肺组织IgE、TNF-α含量显著降低(P<0.01),而TGFβ1含量无统计学差异(P>0.05);平喘汤中剂量组大鼠肺组织IgE、TGFβ1含量高于博利康尼组(P<0.05),而TNF-α含量无统计学差异(P>0.05);平喘汤低剂量组大鼠肺组织IgE、TGFβ1、TNF-α含量均高于博利康尼组(P<0.01)。平喘汤高剂量组优于低剂量组。结论平喘汤能够通过下调大鼠肺组织IgE、TGFβ1及TNF-α含量,可降低气道高反应性、减轻气道炎症症状、控制或延缓气道纤维化进程。
Objective To investigate the intervention of Pingchuan Tang in bronchial asthma rats. Methods 48 SD rats were randomly divided into normal control group, asthma model group, low-dose group of Pingchuan, medium-dose group of Pingchuan, high-dose group of Pingchuan, and positive control group. The asthma model was induced by ovalbumin sensitization in rats. The rats in the treatment group were given intragastric administration of 2.6 mL / time, 1.3 mL / time and 0.7 mL / dose from the third week of the experiment. , The positive drug (Boli Kang Ni) control group 0.1 mL / sub-dose gavage, the normal group and model group were given 1.3 mL / saline, once a day for 4 weeks. The changes of IgE, TGFβ1 and TNF-α in the lungs of rats were observed. Results The levels of IgE, TGFβ1 and TNF-α in the asthmatic group and each treatment group were significantly higher than those in the normal group (P <0.05). The contents of IgE, TGFβ1 and TNF-α in the lung tissue in each treatment group were lower (P <0.05). The content of IgE and TNF-α in the lung tissue of high-dose Pingchuan decoction group was significantly lower than that of the bollocix group (P <0.01), while the content of TGFβ1 (P> 0.05). The content of IgE and TGFβ1 in the lung tissue of middle-dose group of Pingchuan decoction group was higher than that of the balloon group (P <0.05), while the content of TNF-α had no statistical difference (P> 0.05). The levels of IgE, TGFβ1 and TNF-α in lung tissue of low-dose Pingchuan decoction group were higher than those of the BoliCan group (P <0.01). Pingchuan high-dose group than the low-dose group. Conclusion Pingchuan Decoction can reduce the airway hyperresponsiveness, reduce the symptoms of airway inflammation, and control or delay the process of airway fibrosis by down-regulating the content of IgE, TGFβ1 and TNF-α in rat lung tissue.