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目的 血管内皮生长因子(VEGF)与实体瘤的发生、发展和预后相关,但其与儿童急性白血病的 关系尚不明确。本实验通过检测VEGF及其受体fms样酪氨酸激酶受体(Flt 1)及含激酶插入区受体(KDR)在儿 童急性白血病的表达情况,分析它们与儿童急性白血病的发生与预后的关系,为进一步研究抗白血病治疗新靶点 提供思路。方法 采用RT PCR法检测21例初发和复发、20例缓解后白血病患儿和5例健康儿童骨髓单个核细 胞VEGF、Flt 1、KDRmRNA的表达。使用酶联免疫吸附法检测上述患儿及20例正常儿童外周血VEGF蛋白浓度。 结果 健康儿童骨髓单个核细胞均未检测到VEGF及其受体Flt 1,KDR的表达。90%(19/21)初发/复发白血病 患儿骨髓单个核细胞表达VEGF,86%(18/21)表达Flt 1,30%(6/20)缓解后白血病患儿骨髓单个核细胞表达 VEGF,15%(3/20)表达Flt 1,两组差异有显著性(均P<0.001)。初发/复发组VEGF和Flt 1阳性率与正常组 [0%(0/5);0%(0/5)]比较差异有显著性(均P<0.001),而缓解组与正常组比较差异无显著性。两组白血病患 儿未检测到KDR表达。初发/复发组血浆VEGF浓度为405±270pg/mL,高于缓解组(136±98pg/mL,P<0.01) 和正常组(91±41pg/mL,P<0.01)。缓解组与正常组比较差异无显著性。结论 白血病患儿表达VEG
Objective Vascular endothelial growth factor (VEGF) is associated with the occurrence, development and prognosis of solid tumors, but its relationship with childhood acute leukemia is not yet clear. In this study, VEGF and its receptor fms like tyrosine kinase receptor (Flt 1) and kinase-containing receptor (KDR) in children with acute leukemia expression, analysis of their association with the occurrence and prognosis of childhood acute leukemia Relationship, provide ideas for further research on anti-leukemia treatment of new targets. Methods RT-PCR was used to detect the expression of VEGF, Flt 1 and KDR mRNA in 21 cases of primary and recurrent, 20 cases of post-leukemia and 5 cases of healthy children. Enzyme-linked immunosorbent assay was used to detect the concentration of VEGF protein in peripheral blood of the above children and 20 normal children. Results The expression of VEGF and its receptors Flt 1 and KDR in bone marrow mononuclear cells of healthy children were not detected. 90% (19/21) of patients with primary / recurrent leukemia had bone marrow mononuclear cells expressed VEGF, 86% (18/21) express Flt 1, 30% (6/20) of children with leukemia after remission of bone marrow mononuclear cells expressed VEGF , 15% (3/20) express Flt 1, the difference between the two groups was significant (P <0.001). The positive rates of VEGF and Flt1 in primary / relapse group were significantly different from those in normal group [0% (0/5); 0% (0/5)] (P <0.001) No significant difference. KDR expression was undetectable in both groups of leukemias. Plasma VEGF concentration in the initial / relapse group was 405 ± 270pg / mL, higher than that in the remission group (136 ± 98pg / mL, P <0.01) and normal group (91 ± 41pg / mL, P <0.01). There was no significant difference between remission group and normal group. Conclusion The expression of VEG in children with leukemia