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目的探讨三乙胺对永生化人支气管上皮细胞(HBE)的氧化损伤作用。方法体外培养HBE细胞,在6个不同染毒浓度(0、5、7.5、10、15、20mmol/L)和4个不同染毒时间段(6、12、24、48h)的条件下染毒HBE细胞,CCK-8试剂盒检测三乙胺对HBE细胞存活率的影响;收集细胞后装载探针检测细胞活性氧(ROS)的含量;同时通过单细胞凝胶电泳试验(SCGE)检测细胞DNA链断裂情况。结果在相同染毒时间段内,HBE细胞的存活率随着染毒浓度的增加而下降,各浓度组与对照组的差异有统计学意义(P<0.05),且呈明显的剂量-效应关系;随着染毒浓度的增加,HBE细胞ROS含量明显上升,与相同染毒时间段对照组比较,差异有统计学意义(P<0.05);SCGE实验结果表明,各组HBE细胞Olive尾距随染毒浓度增加明显增加,呈现明显的剂量—反应关系,与相同染毒时间段对照组比较,差异有统计学意义(P<0.05)。结论三乙胺可降低HBE细胞存活率,诱导HBE细胞产生自由基从而引起DNA损伤。
Objective To investigate the effect of triethylamine on the oxidative damage of immortalized human bronchial epithelial cells (HBE). Methods HBE cells were cultured in vitro and exposed to 6 different concentrations (0, 5, 7.5, 10, 15 and 20 mmol / L) and 4 different exposure periods HBE cells and CCK-8 kit were used to detect the effect of triethylamine on the survival rate of HBE cells. After the cells were collected, the cells were loaded with probe to detect the content of reactive oxygen species (ROS). Meanwhile, the single cell gel electrophoresis (SCGE) Chain fracture situation. Results During the same exposure time, the survival rate of HBE cells decreased with the increase of exposure concentration, and there was a significant difference between each concentration group and the control group (P <0.05), and showed a dose-response relationship ; With the increase of exposure concentration, the content of ROS in HBE cells increased significantly compared with the control group in the same exposure time (P <0.05). The results of SCGE showed that the change of Olive distance There was a significant dose-response relationship between the exposure and the exposure time. The difference was statistically significant (P <0.05). Conclusion Triethylamine can reduce the survival rate of HBE cells and induce the production of free radicals in HBE cells to cause DNA damage.