【摘 要】
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Objective:Exercise,as a common non-drug intervention,is one of several lifestyle choices known to reduce the risk of cancer.Mitochondrial division has been reported to play a key role in the occurrence and transformation of hepatocellular carcinoma (HCC).
【机 构】
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school of Traditional Chinese Medicine,Naval Medical University,Shanghai 200433,China;Nanjing Univer
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Objective:Exercise,as a common non-drug intervention,is one of several lifestyle choices known to reduce the risk of cancer.Mitochondrial division has been reported to play a key role in the occurrence and transformation of hepatocellular carcinoma (HCC).This study investigated whether exercise could regulate the occurrence and development of HCC through mitosis.Methods:Bioinformatics technology was used to analyze the expression level of dynamin-related protein 1 (DRP1),a key protein of mitochondrial division.The effects of DRP1 and DRP1 inhibitor (mdivi-1) on the proliferation and migration of liver cancer cells BEL-7402 were observed using cell counting kit-8,plate colony formation,transwell cell migration,and scratch experiments.Enzyme-linked immunosorbent assay,Western blot and real-time polymerase chain reaction were used to detect the expression of DRP1 and its downstream phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) pathway.A treadmill exercise intervention was tested in a nude mouse human liver cancer subcutaneous tumor model expressing different levels of DRP1.The size and weight of subcutaneous tumors in mice were detected before and after exercise.Results:The expression of DRP1 in liver cancer tissues was significantly upregulated compared with nor-mal liver tissues (P ＜ 0.001).The proliferation rate and the migration of BEL-7402 cells in the DRP1 over-expression group were higher than that in the control group.The mdivi-1 group showed an inhibitory effect on the proliferation and migration of BEL-7402 cells at 50 μmol/L.Aerobic exercise was able to inhibit the expression of DRP1 and decrease the size and weight of subcutaneous tumors.Moreover,the expression of phosphorylated PI3K (p-PI3K) and phosphorylated AKT (p-AKT) decreased in the exer-cise group.However,exercise could not change p-PI3K and p-AKT levels after knocking down DRP1 or using mdivi-1 on subcutaneous tumor.Conclusion:Aerobic exercise can suppress the development of tumors partially by regulating DRP1 through PI3K/AKT pathway.
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