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叶序和出叶间隔期是叶片生长发育的基本生物学特性和水稻的重要农艺性状之一。对叶序或出叶间隔期突变体的研究,可以帮助我们了解叶片的形成机制。本研究通过甲基磺酸乙酯(EMS)诱变粳稻品种日本晴,获得一个稳定遗传的类树状突变体s2-21。该突变体出叶间隔期变短、节间缩短、植株矮化、分蘖数减少、叶片数增加、不能正常进行生殖生长。将该突变体与籼稻品种Dular杂交,遗传分析表明该突变体性状受1对隐性基因控制。通过InDel分子标记对s2-21/Dular F2群体进行遗传定位,将该基因初步定位在第1染色体InDel标记C1-15和S1-17之间。利用本实验已测序的籼稻品种Dular全基因组序列与NCBI(http://www.ncbi.nlm.nih.gov/)上提供的粳稻品种日本晴基因组序列比对,开发了6个新的InDel标记,最终将该基因定位在W25和W26之间约88 kb的区间内。测序结果表明该突变体中PLA2基因的第4个内含子的第5位碱基由G突变为A。
Leaf sequence and leaf interval is the basic biological characteristics of leaf growth and development and one of the important agronomic traits in rice. Research on leaf sequence or leaf-out interval mutants can help us understand the mechanism of leaf formation. In this study, a japonica variety Nipponbare was mutagenized by ethyl methanesulfonate (EMS) to obtain a stable inherited dendritic mutant s2-21. The mutant leaves a shorter interval between leaves, internodes shortened, plant dwarfing, tillering decreased, the number of leaves increased, not normal reproductive growth. The mutant was crossed with indica Dular, and the genetic analysis indicated that the trait was controlled by one recessive gene. The s2-21 / Dular F2 population was genetically mapped by InDel molecular markers, and the gene was preliminarily located between chromosomes InDel marker C1-15 and S1-17. Six new InDel markers were developed using the Dular whole genome sequence of indica rice species sequenced in this experiment and the japonica rice genomic Nipponbare sequence provided on NCBI (http://www.ncbi.nlm.nih.gov/) The gene was finally located within the interval of about 88 kb between W25 and W26. The sequencing results showed that the fifth base of the fourth intron of PLA2 gene in this mutant was mutated from G to A.