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以欧洲花楸悬浮培养细胞(SASC)为材料,检测酵母提取物(YE)处理后SASC生理生化指标的变化,初步探究YE诱导SASC合成次生代谢产物的机制。结果表明:YE诱导SASC合成了5种联苯类化合物,且5种化合物含量随诱导时间的延长呈现不同的变化规律;YE处理抑制细胞的生长;细胞培养液中的pH随着处理时间的延长逐渐降低;细胞中的可溶性蛋白质含量表现为缓慢下降的变化趋势,YE处理组(YE组)可溶性蛋白质含量与对照组(CK组)相比显著增加,最高时相对增量达到了147.76%;CK组和YE组细胞外Ca2+都表现为内流,但YE组的内流明显小于CK组。说明YE诱导使细胞处于一种胁迫状态,不利于细胞的生长,迫使细胞合成了联苯类化合物抵御外界胁迫;细胞内可溶性蛋白可能作为酶类参与调控化合物的合成;Ca2+信号分子可能介导细胞应对YE胁迫的信号转导。
SASC was used as a material to detect the changes of physiological and biochemical indexes of SASC after yeast extract (YE) treatment, and to explore the mechanism of YE-induced SASC synthesis of secondary metabolites. The results showed that YE induced SASB synthesis of five biphenyl compounds, and the five compounds showed different changes with the induction time; YE treatment inhibited the growth of cells; the pH of the cell culture medium with the extension of treatment time (P <0.05). The content of soluble protein in the cells showed a slowly decreasing trend. The content of soluble protein in YE treatment group (YE group) increased significantly compared with the control group (CK group), reaching a maximum of 147.76% The extracellular Ca2 + in group and YE group showed influx, but the inflow of YE group was significantly less than CK group. Indicating that YE induced cells in a state of stress is not conducive to cell growth, forcing the synthesis of biphenyl compounds against external stress cells; intracellular soluble protein may be involved in the regulation of compounds as enzymes; Ca2 + signaling molecules may mediate cell Response to YE stress signal transduction.