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目的:将体外构建的组织工程右心带瓣管道,以带瓣补片的形式移植于犬主肺动脉,观测带瓣管道材料体内情况。方法:去细胞处理牛颈静脉体,无菌处理后种植标记过的犬骨髓间质干细胞,构建组织工程带瓣管道,犬开胸手术,将体外构建的组织工程右心带瓣管道,以带瓣补片的形式移植于犬主肺动脉,术后4、8、12行胸部B超检查;取出补片,HE染色;荧光显微镜下标记细胞检测;样本钙含量测定。结果:术后犬胸部B超观察:瓣叶无增厚,钙化,管道血流通畅,无血栓及钙化。术后4、8、12周除了瓣叶逐渐缩小外,补片无动脉瘤形成,瓣膜表面光滑,无血栓形成,弹性良好,血管壁内面光滑,无血栓形成。种植种子细胞牛颈静脉带瓣补片成活。4周钙含量增加,8周时候,钙含量又有增加,12周时钙含量与8周相比无明显变化。结论:组织工程技术构建组织工程右心带瓣管道有可行之处。
OBJECTIVE: To transplan the tissue-engineered right heart valve flap constructed in vitro into the pulmonary artery of the canine by means of valved patch, and to observe the in vivo condition of the flap material. Methods: The bovine jugular vein was decellularized and labeled with canine bone marrow mesenchymal stem cells after aseptic treatment. Tissue engineered flap and canine thoracotomy were constructed. Tissue engineered right ventricular valve was constructed in vitro. Flap patch transplanted in the main pulmonary artery, 4,8,12 postoperative chest B-ultrasound; remove the patch, HE staining; fluorescence microscopy labeled cells; sample calcium content determination. Results: Postoperative B-thoracic B-observation: no thickening of valve leaflets, calcification, smooth blood flow in the duct, no thrombosis and calcification. At 4, 8, and 12 weeks after operation, there was no aneurysm formation in the patch, and the valve surface was smooth, thrombus-free, and elastic was good. The inner surface of the vessel wall was smooth and thrombus-free. Planting seeds cell bovine jugular vein valve flap survival. After 4 weeks, the calcium content increased. After 8 weeks, the calcium content increased again. At 12 weeks, the calcium content did not change significantly compared with the 8th week. Conclusion: It is feasible to construct tissue engineering right ventricular valve with tissue engineering.