本研究应用逆转录聚合酶链反应技术（ＲＴ－ＰＣＲ）检测脑脊液及血清中柯萨奇病毒的ＲＮＡ。４３例临床确诊的病毒性脑膜炎患者中有１７例脑脊液ＲＴ－ＰＣＲ检查阳性，血清有１３例阳性；而对照组２１例其它中枢神经系统疾病的脑脊液及血清均为阴性。用柯萨奇病毒引物扩增其它病毒，结果也为阴性。因此，很适于进行柯萨奇病毒的早期、快速诊断。 In this study, Coxsackie RNA in cerebrospinal fluid and serum was detected by reverse transcription-polymerase chain reaction (RT-PCR). Of the 43 clinically diagnosed patients with viral meningitis, 17 were positive for CSF RT-PCR and 13 were positive for serum, while 21 of the controls were negative for cerebrospinal fluid and serum from other central nervous system diseases. Other Coxsackie virus primers were used to amplify other viruses and the result was also negative. Therefore, it is suitable for the early, rapid diagnosis of Coxsackie virus.