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利用大鼠肠系膜上动脉夹闭造成缺血再灌流动物模型，用原位杂交和ＲＴ－ＰＣＲ技术检测肺内源性ｂＦＧＦ和ＴＧＦβ基因表达的变化。通过观察肠缺血再灌流损伤后肺内源性ｂＦＧＦ和ＴＧＦβ基因表达的变化规律，探讨这两种生长因子与肺损伤修复的关系。结果：在正常肺泡上皮细胞和微静脉中，ｂＦＧＦ和ＴＧＦβ均有一定表达。缺血４５ｍｉｎ时，ＴＧＦβ表达量显著增加，而ｂＦＧＦ无明显改变。再灌流６ｈ后，两种因子基因表达水平均明显增高，而再灌流２４ｈ两种因子基因表达量接近于伤前。结果表明，肠缺血再灌流导致肺ｂＦＧＦ和ＴＧＦβ基因表达水平呈规律性变化，并且这种变化与肺损伤和修复过程密切相关，表明肺在损伤后具主动修复过程，提示这可能是肺的自身保护机制之一。
The ischemia-reperfusion animal model was established by clamping the superior mesenteric artery in rats. The expression of endogenous bFGF and TGFβ gene in lung tissues was detected by in situ hybridization and RT-PCR. By observing the changes of endogenous bFGF and TGFβ gene expression after intestinal ischemia-reperfusion injury, we explored the relationship between these two growth factors and lung injury repair. Results: In normal alveolar epithelial cells and venules, both bFGF and TGFβ were expressed. At 45min ischemia, TGFβ expression increased significantly, while bFGF had no significant change. After reperfusion for 6h, the expression levels of two factors were significantly increased, while the expression of two genes after reperfusion 24h was close to pre-injury. The results showed that intestinal ischemia-reperfusion led to a regular change of lung bFGF and TGFβ gene expression levels, and this change is closely related with the process of lung injury and repair, indicating that lung repair with active repair process, suggesting that this may be the lung One of its own protection mechanisms.