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目的:初步探讨花生茎叶镇静催眠的活性部位及作用机制。方法:观察花生茎叶水提物(10、30、100 g/kg)对小鼠自主活动和阈下剂量戊巴比妥钠致小鼠睡眠的影响,确定花生茎叶水提物有效剂量。将花生茎叶水提物进一步用石油醚、乙酸乙酯和正丁醇依次提取,得到极性由小到大的3个提取部位,分别观察花生茎叶上述提取部位对小鼠自主活动和阈下剂量戊巴比妥钠致小鼠睡眠的影响,以确定有效部位;观察花生茎叶不同提取部位对小鼠脑内单胺类递质的影响,以初步探讨其镇静催眠作用机制。结果:花生茎叶水提物(生药100 g/kg)能减少小鼠自主活动次数,增加阈下剂量戊巴比妥钠致小鼠入睡率;花生茎叶石油醚部位和乙酸乙酯部位能显著减少小鼠自主活动次数、增加阈下剂量戊巴比妥钠致小鼠入睡率,升高小鼠脑内5-HT含量。结论:花生茎叶的石油醚部分和乙酸乙酯部位是镇静催眠作用的有效部位,其镇静催眠作用机制可能与提高小鼠脑内5-HT水平有关。
Objective: To investigate the active site and mechanism of sedation and hypnosis in peanut stems and leaves. Methods: The effects of aqueous extract of stems and leaves of peanut (10, 30, 100 g / kg) on the mice sleep induced by spontaneous activity and subthreshold dose of pentobarbital were observed, and the effective dose of aqueous extract of stems and leaves of peanut was determined. The aqueous extract of peanut stem and leaf was further extracted with petroleum ether, ethyl acetate and n-butanol successively to obtain three parts with small to large polarities. The extraction sites of the peanut stem and leaves were observed respectively on autonomic activity and subthreshold Dose pentobarbital sodium in mice to determine the effective site to observe the effect of different extraction sites of peanut on the monoamine neurotransmitters in mice brain to explore its mechanism of sedative and hypnotic effects. Results: The aqueous extract of stems and leaves of peanut (100g / kg crude drug) could reduce the frequency of spontaneous activity in mice and increase the rate of falling asleep in mice induced by subthreshold dose of pentobarbital sodium. The content of petroleum ether and ethyl acetate in peanut Significantly reduce the frequency of spontaneous activity in mice, increase the subliminal dose of pentobarbital sodium in mice to fall asleep, and increase the content of 5-HT in mice brain. CONCLUSION: The petroleum ether fraction and ethyl acetate fraction of peanut stem and leaves are effective sites for sedative and hypnotic effects. The mechanism of sedative and hypnotic effects may be related to increasing the 5-HT level in brain of mice.