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我们首次采用标记和定位抗脑抗体(ABA)抗血清,观察其在听源性癫痫大鼠(GEPRs)不同脑区和神经结构中的分布,结合组织病理学观察,间接定位与导致痫性放电有关的及附着有ABA的特殊脑抗原结构部位(即癫痫抗原,EPI-AG),直接从免疫学角度揭示癲痫(EPI)发病机制.结果显示,抽搐组GEPRs的额顶叶深层皮质、海马回、齿状回、丘脑、小脑颗粒细胞层、丘脑之室管膜及海马之软脑膜抽搐或抽搐预激敏感脑区呈ABA阳性反应.其中又以纤维型星形胶质细胞突及其分枝末端最明显.组织病理学结果也显示ABA阳性脑区中有活跃的纤维型星形胶质细胞.因此,纤维型星形胶质细胞成份,不仅参与EPI免疫发病的主要过程,还可能做为EPI-AG引发EPI免疫反应,从而导致痫性放电甚或临床发作.
For the first time, we labeled and localized anti-brain antibody (ABA) antiserum and observed its distribution in different brain regions and neural structures of auditory epileptic rats (GEPRs). Combined with histopathological observation, indirect localization and epileptiform discharge (Epilepsy antigen, EPI-AG), which is related to the specific brain antigen structure (ie EPI-AG) attached to ABA, revealed the pathogenesis of epilepsy (EPI) directly from the perspective of immunology.The results showed that the frontal lobe cortex and hippocampus Back, dentate gyrus, thalamus, cerebellar granule cell layer, the thalamus ependymal and hippocampal pial seizures or convulsions pre-excited sensitive brain area was ABA-positive reaction which in order to fiber type astrocyte processes and its points The most obvious branch and end.The histopathological results also show that there are active fibroblastic astrocytes in the ABA-positive brain region.Therefore, fibrillar astrocyte components, not only involved in the main process of EPI immunization, may also be done EPI-AG triggered EPI immune response, leading to epileptic discharge or even clinical attack.