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盐度是与渗透压相关的重要环境因子,与水生生物的生存与进化密切相关.为探索魁蚶对低盐的适应能力与渗透压调节机制,本文研究了不同盐度(10,15,20,25)胁迫下,魁蚶(Scapharca broughtonii)的存活情况,以及相应的肝胰腺、外套膜、鳃、斧足、闭壳肌等五种组织中Na~+/K~+-ATP酶活性的变化.结果表明:盐度越低,魁蚶出现死亡的时间越早,半致死浓度(LD50)越大;24,48,72,96 h的LD50分别为5.790,9.872,14.463,14.894.随着盐度不断降低和处理时间延长,Na~+/K~+-ATP酶活力总体呈先上升后下降趋势(p<0.05),适当降低水体盐度可激活并增强魁蚶体内Na~+/K~+-ATP酶活力,但Na~+/K~+-ATP酶被激活具有组织器官特异性和时序性,且超过盐度耐受限度,酶活反被抑制.本研究探索了低盐对魁蚶存活及体内Na~+/K~+-ATP酶的影响,明确了魁蚶对低盐的耐受能力和自身调节生理过程,进一步丰富魁蚶基础研究资料,为培育魁蚶耐低盐新品系提供了科学依据,对于发展和促进魁蚶低盐模式具有重要科学意义.
Salinity is an important environmental factor related to osmotic pressure and closely related to the survival and evolution of aquatic organisms.In order to explore the mechanism of adaptability and osmotic regulation of low-salt aquatic organisms, salinity was studied in different salinities (10, 15, 20 , 25), the survival of Scapharca broughtonii, and the relative activities of Na ~ + / K ~ + -ATPase in five tissues including hepatopancreas, mantle, gills, The results showed that the lower the salinity, the earlier the death occurred and the higher the LD50, and the LD50 at 24, 48, 72 and 96 h were 5.790, 9.872, 14.463 and 14.894, respectively Na + / K + -ATPase activity increased first and then decreased (p <0.05), and salinity decreased and the treatment time prolonged. The activity of Na ~ + / K ~ + -ATP enzyme activity, but Na ~ + / K ~ + -ATPase was activated with tissue-organ specific and time-dependent, and beyond the limits of salinity tolerance, enzyme activity was inhibited.In this study, Kui survival and in vivo Na ~ + / K ~ + -ATP enzyme, Kui clear Kui tolerance to low salt and regulate their own physiological processes to further enrich Kui basic research data for the training Kui蚶 low resistance to salt new strains provide a scientific basis for the development and promotion Kui 蚶 low salt model has important scientific significance.