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目的:考察川木香煨制前后木香烃内酯(costunolide,COS)和去氢木香内酯(dehydrocostus lactone,DEH)在人工胃、肠液中的稳定性,及其在大鼠离体胃、小肠、结肠共孵液中的吸附、摄取或降解特征。方法:采用高效液相色谱法测定川木香煨制前后COS和DEH在人工胃、肠液及大鼠离体胃肠共孵液中的质量浓度,以大鼠离体胃、小肠、结肠与药物共孵的方法考察其物理吸附、摄取或降解参数。结果:川木香煨制前后COS在人工胃液稳定性较差,平均降解常数分别为0.758 0,0.531 1,煨制后稳定性有所提高,具统计学意义(P<0.01);川木香煨制前后COS和DEH在大鼠离体胃、小肠和结肠中的吸附、摄取或降解量(2 h)均较大,其中煨制前后COS和DEH在不同部位均有不同程度的差异。结论:川木香煨制前后COS在人工胃液中均不稳定,煨制后稳定性有所提高;大鼠离体胃、小肠、结肠对川木香煨制前后的COS和DEH均有明显的吸附、摄取或降解,并在不同部位有差异;为川木香煨制前后药效成分的吸收机制研究提供了参考。
OBJECTIVE: To investigate the stability of costunolide (COS) and dehydrocostus lactone (DEH) in artificial stomach and intestine before and after dawn simmered, , Intestinal, colon co-incubation fluid adsorption, uptake or degradation characteristics. Methods: The contents of COS and DEH in artificial stomach, intestinal juice and rat gastrointestinal co-incubation solution were determined by HPLC before and after simvastatin. The gastric, intestinal, colon and drug Methods of incubation were investigated for physical adsorption, uptake or degradation parameters. Results: The stability of COS in artificial gastric juice was poor before and after the treatment with Chuanmusiangao, with the average degradation constant of 0.758 0 and 0.531 1, respectively. The stability of simvastatin was improved with statistical significance (P <0.01) Before and after simmering, COS and DEH adsorbed, ingested or degraded in the isolated stomach, small intestine and colon (2 h), respectively. Both COS and DEH had different degrees in different parts before and after simmering. Conclusion: COS was not stable in artificial gastric juice before and after cooking, but the stability after simmering was improved. COS and DEH in rats’ stomach, small intestine and colon before and after simmering were significantly Adsorption, ingestion or degradation, and in different parts of the difference; for Sichuan wood simmer before and after the medicinal composition of the absorption mechanism of study provides a reference.