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【目的】构建含有 ap2启动子/增强子序列、AQP7 cDNA 的融合蛋白表达载体 pEGFP-ap2-AQP7。【方法】将 ap2增强子和启动子和 AQP7 cDNA拼接后产物克隆到 pIRES2-EGFP上，与腺病毒载体重组获得的融合蛋白，转染3T3脂肪细胞测定侵染效果。【结果】①构建的细胞株稳定表达 pEGFP-ap2-AQP7融合蛋白；②使用增强型绿色荧光蛋白作为标记物，可以观察到 AQP7在3T3脂肪细胞的定位。【结论】稳定表达 pEGFP-ap2-AQP7融合蛋白的3T3脂肪细胞株，为 AQP7在细胞内定位与功能研究奠定了基础。“,”[Objective]To construct fusion protein expression vector pEGFP-ap2-AQP7 of promoter/enhancer sequences of ap2 and AQP7 cDNA.[Methods]The products of splicing promoter/enhancer of ap2 and AQP7 cDNA were cloned into pIRES2-EGFP.Adenovirus vector recombinant fusion protein was obtained.The infected 3T3 fat cell was used to determine the infextation effect.[Results]① The pEGFP-ap2-AQP7 fusion protein was expressed in the constructed cell strains stably.The enhanced green fluorescent protein was used as a marker.The positio-ning of AQP7 in 3T3 fat cells were observed.[Conclusion]The stable construction of 3T3 fat cell strains express-ing fusion protein pEGFP-ap2-AQP7 can provide the basis for intracellular localization and function research of AQP7 .