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目的探讨不同处理方法对冷凝集标本血细胞分析的影响。方法分别采用手心揉搓并颠倒混匀2 min(方法 1),37℃水浴30 min(方法 2),血浆置换3次(方法 3),显微镜手工计数(方法 4)处理10例冷凝集标本,比较各种纠正方法对红细胞各参数结果的影响。结果以方法 4检测结果为参比,方法 1对RBC、HCT、MCH、MCHC的检测造成严重干扰,RBC和HCT结果明显降低,MCH和MCHC结果明显增高;方法 2(37℃水浴30 min从水浴箱取出后要进行保温)各参数检测结果与方法 4之间的差异无统计学意义;方法 3(置换血浆3次法)RBC、HCT、MCH、Hb和MCHC与方法 4之间的差异无统计学意义,但PLT明显降低(P<0.05)。结论血常规检验中冷凝集标本推荐采用37℃水浴30 min后保温立即检测,大部分冷凝集标本通过该法均能获得满意的效果;对于极少数较为严重的冷凝集标本,可采用血浆置换3次处理后再检测,但血浆置换易导致血小板的严重丢失,因此,PLT值采用水浴法的检测结果较为准确。
Objective To investigate the influence of different treatment methods on the blood cell analysis of condensed samples. Methods Ten samples of condensed samples were treated by rubbing them with their hands and inverting them for 2 min (Method 1), 37 ° C in water bath for 30 min (Method 2), plasma exchange 3 times (Method 3) and microscope manual counting (Method 4) Various correction methods on the parameters of erythrocytes results. The results of the method 4 test results as a reference, Method 1 of RBC, HCT, MCH, MCHC detection caused serious interference, RBC and HCT results were significantly lower, MCH and MCHC results were significantly increased; Method 2 (37 ℃ water bath 30 min from the water bath There was no significant difference between the detection results of the parameters and the method 4; and the difference between the RBC, HCT, MCH, Hb, and MCHC and the method 4 was not statistically significant by the method 3 (plasma exchange 3 times method) Significance, but PLT was significantly lower (P <0.05). CONCLUSION: The coagulation samples in the routine blood test are recommended to be tested immediately after incubation at 37 ℃ for 30 min, and most of the samples with cold agglutination can obtain satisfactory results by this method. For the very few severe coagulation samples, plasma exchange 3 Secondary treatment and then test, but plasma replacement easily lead to severe platelet loss, therefore, PLT value using water bath test results more accurate.