目的　了解广东省 1996～ 2 0 0 0年钩端螺旋体病人群及宿主动物带菌情况 ,以便采取相应措施确保钩端螺旋体病防治工作顺利开展。方法　采取血培养、动物脏器培养做病原学分离 ,血清学检测采用显微镜凝集试验 (MAT)进行。结果　从病人血及动物脏器中分离出 8株钩端螺旋体 ,鉴定了 6株 ,分属 4种菌群 ,分别为爪哇、秋季、犬热及赛罗群 ;疑似病人血清、健康人血清、宿主动物血清平均抗体阳性率分别为 10 .47%、34 .2 3%、18.2 3%。结论　广东省人群钩端螺旋体隐性感染水平较高 ,在一定程度上起了免疫保护作用 ;首次分离出赛罗群钩端螺旋体 ,在防治工作中应注意菌群变化 Objective To understand the population of Leptospirosis patients and host animals in Guangdong Province from 1996 to 2000 in order to take corresponding measures to ensure the success of prevention and control of leptospirosis. Methods Blood culture and animal organ culture were used for etiological isolation. Serological tests were performed by microscopic agglutination test (MAT). Results Eight strains of Leptospira were isolated from the blood and animal organs of the patients, and six strains were identified, which belonged to four species, namely Java, autumn, canine fever and Siro group. Suspected patient serum, healthy human serum, The average antibody positive rates of host animals were 10.47%, 34.23% and 18.23% respectively. Conclusion Leptospira latent infection in Guangdong population is relatively high, which may play an immunoprotective role to a certain extent. For the first time, the Leptospira interrogans