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以华灰莉木为试材,采用溶剂冷浸法提取植物成分,溶剂液-液萃取法对植物成分进行初步分离,Potter喷雾法测定杀螨活性,研究华灰莉木提取物和初步分离物对柑橘全爪螨的影响。结果表明:华灰莉木叶和枝条甲醇提取物对柑橘全爪螨雌成螨有较高的触杀毒力,致死中浓度(LC50)分别为5.5334g/L和3.9682g/L。叶的杀螨活性物质主要集中在水萃取物中,属于极性强的物质;枝条的杀螨活性物质主要集中在石油醚萃取物中,属于极性弱的物质。叶提取物的水萃取物和枝条提取物的石油醚萃取物对柑橘全爪螨雌成螨的LC50分别为3.2970g/L和1.7372g/L。
In this study, the components of plant were extracted by cold soaking with solvent, the plant components were separated by solvent liquid-liquid extraction, the acaricidal activity was determined by Potter spray method, Effect on Citrus red mite. The results showed that the methanol extracts of leaves and branches of S. auriculata had higher contact toxicity to adult female mites of the citrus red mite, with LC50 of 5.5334 g / L and 3.9682 g / L, respectively. Leaf acaricidal active substances are mainly concentrated in water extracts, belong to strong polar substances; branches of acaricidal active substances are mainly concentrated in petroleum ether extract, belong to the weak polarity of the material. The LC50 of petroleum extracts of leaves extracts and petroleum ether extracts of shoots extracts to adult citrus red mites were 3.2970g / L and 1.7372g / L, respectively.