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对雪腐格氏霉(Gerlachianivalis)产毒培养基的筛选结果表明,适宜菌株生长和产毒的液体培养基为马铃薯蔗糖培养液(PSB)和添加1%蛋白胨的察氏(Czapek’s)培养液。固体培养物玉米粒上的粗毒素产量远高于液体培养基。适宜茵株生长的条件是20℃,pH6~7,黑暗和静止培养,适宜产毒的条件是10℃,pH7~8,光暗交替和静止培养;培养滤液和从固体培养物中浸提的粗毒素对小麦种子均有毒性,浸提的粗毒素的毒性远高于培养滤液。粗毒素对小麦胚根生长的抑制作用与浓度密切相关,与生长率的相关系数为─0.8677(P<0.01)。
Screening of Germfree Gerdchianivalis virulence media showed that suitable bacterial growth and toxigenic liquid media were potato Cultures (PSB) and Czapek’s supplemented with 1% peptone liquid. Crude toxin production on solid cultures of corn is much higher than that of liquid medium. Suitable conditions for the growth of strains are 20 ℃, pH6 ~ 7, dark and stationary culture, the appropriate conditions for the production of toxin is 10 ℃, pH7 ~ 8, light and dark alternation and stationary culture; filtrate and solid culture extract Crude toxins are toxic to wheat seeds and the toxicity of crude toxins extracted is much higher than that of culture filtrate. The inhibitory effect of crude toxin on the growth of wheat radicle was closely related to the concentration, and the correlation coefficient with growth rate was -0.8677 (P <0.01).