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采用TCBS选择培养基从广西钦州市、防城港市等地的凡纳滨对虾及养殖水体中分离纯化得到8株细菌,对分离菌株进行生理生化特性鉴定,并对全部菌株16S rRNA基因序列及部分菌株的HSP60基因序列进行克隆、测序,运用Mega 4.1软件中的Neighbor-Joining法构建系统进化树。选取河流弧菌、溶藻弧菌、哈氏弧菌各1株对凡纳滨对虾成虾进行人工感染试验。结果表明,菌株S被鉴定为河流弧菌,菌株M1、M2、M3、水1、水2、水3被鉴定为溶藻弧菌、菌株H1被鉴定为哈氏弧菌;溶藻弧菌对对虾成虾的致病性最强,其次是哈氏弧菌。
Eight strains of bacteria were isolated and purified from shrimp and culture water of Litopenaeus vannamei in Qinzhou city and Fangchenggang city of Guangxi by TCBS selection medium. The physiological and biochemical characteristics of the isolated strains were identified. The sequences of 16S rRNA gene and some strains The HSP60 gene sequence was cloned and sequenced. The phylogenetic tree was constructed using Neighbor-Joining method in Mega 4.1 software. Vibrio cholerae, Vibrio alginolyticus and Vibrio harveyi were selected to test the artificial infection of Litopenaeus vannamei shrimp. The results showed that strain S was identified as Vibrio cholerae, strains M1, M2, M3, water 1, water 2 and water 3 were identified as Vibrio alginolyticus and strain H1 was identified as Vibrio harveyi. Prawn shrimp is the most pathogenic, followed by Vibrio harveyi.