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登革病毒依抗原性不同,可分为1、2、3、4四个血清型。它除了能导致一定范围内发热的典型登革热外,还能引致更严重的登革出血热和登革休克综合症。由于没有具体有效的治疗药物,目前尚无好的治疗登革热的方法,治疗方案多是支持性的。本实验评估了在2型登革病毒感染的人血源性巨噬细胞中沙棘叶提取物的抗登革病毒活性,选择巨噬细胞的原因是因为巨噬细胞是登革病毒感染的首要目标。受感染的细胞用沙棘叶提取物治疗并与市售的抗病毒药物利巴韦林作比较,研究发现沙棘叶提取物维持登革病毒感染细胞活力的能力几乎等同于利巴韦林。传统的空斑试验进一步确定了沙棘叶提取物的抗登革病毒活性。这些试验结果表明,沙棘叶提取物具有显著的抗登革病毒活性及治疗登革热的潜力。
Dengue virus according to antigenicity can be divided into 1,2,3,4 four serotypes. Apart from the typical dengue fever that causes a certain range of fever, it can lead to more severe dengue hemorrhagic fever and dengue shock syndrome. There is no good way to treat dengue because there is no specific and effective treatment, and most of the treatment options are supportive. This experiment evaluated the anti-dengue virus activity of seabuckthorn leaf extract in human blood-derived macrophages infected with type 2 dengue virus. The reason for choosing macrophages is that macrophages are the primary target of dengue virus infection . Infected cells were treated with sea-buckthorn leaf extract and compared with the commercially available antiviral ribavirin. The study found that the seabuckthorn leaf extract’s ability to maintain dengue virus-infected cells was almost equivalent to ribavirin. Traditional plaque test further confirmed the anti-dengue virus activity of seabuckthorn leaf extract. These test results show that the seabuckthorn leaf extract has significant anti-dengue virus activity and potential for the treatment of dengue fever.