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目的:利用流式细胞仪和基因芯片技术分析比较基因敲入(KIMAP)和转基因(TGMAP)小鼠前列腺癌模型。方法:对于来自KIMAP和TGMAP的肿瘤标本进行流式细胞仪分析和基因芯片分析。结果:来源于TGMAP(n=8)和KIMAP(n=9)的小鼠前列腺癌标本流式细胞仪分析显示,多数KIMAP肿瘤为双倍体,与临床相近,而所有TGMAP肿瘤均为非整倍体。基因芯片分析证明,在KIMAP中免疫应答基因高表达,而TGMAP晚期肿瘤(26～32周)中与神经内分泌肿瘤分化相关的基因占主体。结论:由于KIMAP模型成功地模仿了人类前列腺癌特征,在前列腺癌临床前期研究中具有潜在的应用价值。
OBJECTIVE: To compare the expression of KIMAP and TGMAP in prostate cancer in mice by flow cytometry and microarray. Methods: Tumor specimens from KIMAP and TGMAP were analyzed by flow cytometry and microarray analysis. RESULTS: Flow cytometric analysis of mouse prostate cancer specimens from TGMAP (n = 8) and KIMAP (n = 9) showed that most KIMAP tumors were diploid and similar to the clinical counterpart, whereas all TGMAP tumors were non-whole Ploidy. Microarray analysis demonstrated the high expression of immune response genes in KIMAP, whereas genes associated with neuroendocrine tumor differentiation were predominant in advanced TGMAP tumors (26-32 weeks). Conclusion: Because KIMAP model successfully mimics the characteristics of human prostate cancer, it has potential value in the preclinical study of prostate cancer.