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本研究旨在探究载脂蛋白CⅢ(ApoCⅢ)刺激猪主动脉血管内皮细胞前后的差异基因表达谱,从而揭示ApoCⅢ的功能。利用酶解法成功分离猪主动脉血管内皮细胞并进行体外培养,采用高通量测序技术筛选出ApoCⅢ刺激前后的差异表达基因。结果表明,ApoCⅢ刺激猪主动脉血管内皮细胞前后共有647个差异表达基因,包括390个上调表达基因和257个下调表达基因。实时荧光定量PCR(qRT-PCR)检测表明,高通量测序数据结果正确可靠。GO及Pathway分析结果显示,差异表达基因的功能涉及免疫应答、细胞凋亡及死亡等。这表明ApoCⅢ可通过炎症反应、细胞黏附、细胞凋亡等分子通路影响猪主动脉血管内皮细胞的生理功能,为进一步解析ApoCⅢ引发动脉粥样硬化发生的分子机制提供了理论基础。
The purpose of this study was to investigate the differential gene expression profile of porcine aortic endothelial cells stimulated by apolipoprotein C Ⅲ (ApoC Ⅲ) to reveal the function of ApoC Ⅲ. The porcine aortic endothelial cells were successfully isolated by enzymatic hydrolysis and cultured in vitro. High-throughput sequencing was used to screen the differentially expressed genes before and after ApoCIII stimulation. The results showed that ApoC Ⅲ had a total of 647 differentially expressed genes before and after stimulation of porcine aortic endothelial cells, including 390 up-regulated genes and 257 down-regulated genes. Real-time quantitative PCR (qRT-PCR) tests showed that high-throughput sequencing data was correct and reliable. GO and Pathway analysis showed that the function of differentially expressed genes involved in immune response, apoptosis and death. This indicates that ApoCⅢ can affect the physiological functions of porcine aortic endothelial cells through molecular pathways such as inflammatory reaction, cell adhesion and apoptosis, and provide a theoretical basis for further analysis of the molecular mechanism of ApoC Ⅲ-induced atherosclerosis.