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【目的】观察补阳还五汤对缺氧缺糖(oxygen-glucose deprivation,OGD)损伤PC12细胞凋亡的影响,筛选补阳还五汤中抑制OGD损伤PC12细胞凋亡的主要药味。【方法】将补阳还五汤中7种单味药即黄芪、赤芍、当归尾、地龙、川芎、桃仁、红花作为实验因素,每个因素选取有或无2个水平,按L8(27)正交试验表安排试验。采用OGD细胞模型,运用中药血清药理学方法和流式细胞术技术,以细胞凋亡率为评价指标,利用直观分析和方差分析法,比较空白血清组(有氧+含糖培养基+空白血清)、模型组(缺氧+无糖培养基+空白血清)和补阳还五汤(缺氧+无糖培养基+含药血清)以及各拆方对OGD损伤PC12细胞凋亡的影响。【结果】与空白血清组比较,模型组PC12细胞凋亡率显著增加,差异有统计学意义(P<0.01);与模型组比较,补阳还五汤组的凋亡率显著降低,差异有统计学意义(P<0.01)。正交试验分析显示:补阳还五汤中对OGD损伤PC12细胞凋亡具有显著性影响的药味为桃仁、红花、赤芍(P<0.01),其余药味作用不显著(P>0.05),含赤芍的拆方细胞凋亡率低于不含赤芍的拆方。【结论】补阳还五汤具有抑制OGD损伤PC12细胞凋亡的作用,其中发挥作用的主要药味为赤芍。
【Objective】 To observe the effect of Buyang Huanwu decoction (PCH) on the apoptosis of PC12 cells induced by oxygen-glucose deprivation (OGD), and to screen the main pharmacological action of Buyang Huanwu Decoction inhibiting PC12 cells apoptosis induced by OGD. 【Method】 Seven kinds of single herbs in Buyang Huanwu Decoction, Astragalus, Radix Paeoniae Alba, Angelica sinensis, Dilong, Chuanxiong, peach kernel and safflower were selected as experimental factors. Each factor was selected with or without 2 levels and selected by L8 (27) orthogonal test table arrangement test. Using OGD cell model, using traditional Chinese medicine serum pharmacology method and flow cytometry technology, taking apoptosis rate as evaluation index, comparing with blank serum group (aerobic + sugar-containing medium + blank serum ), Model group (hypoxia + sugar-free medium + blank serum) and Buyang Huanwu Decoction (hypoxia + sugar-free medium + serum containing) and the demolition of OGD PC12 cells. 【Results】 Compared with the blank serum group, the apoptosis rate of PC12 cells in model group was significantly increased (P <0.01), and the apoptosis rate of Buyang Huanwu Decoction group was significantly lower than that in model group Statistical significance (P <0.01). Orthogonal test results showed that Buyi Huanwu Decoction could significantly inhibit the apoptosis of PC12 cells induced by OGD, and the effect was not obvious (P> 0.05) Apoptosis rate of cells with TPG was lower than that without TPG. 【Conclusion】 Buyang Huanwu Decoction can inhibit the apoptosis of PC12 cells induced by OGD, and the main drug that plays a role in it is Radix Paeoniae Rubra.