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目的 :观察白杨素对急性哮喘模型小鼠肺组织核因子(nuclear factor,NF)-κB表达的影响。方法 :24只雌性BALB/c小鼠随机分成4组:正常对照组、哮喘组、白杨素组、布地奈德组,每组6只。使用卵白蛋白腹腔注射致敏,气道激发制备哮喘模型,白杨素组小鼠给予白杨素50 mg/kg灌胃,布地奈德组小鼠给予布地奈德雾化液雾化吸入。肺功能测定评价小鼠气道阻力,HE染色评价小鼠气道炎症,酶联免疫吸附(ELISA)法检测支气管肺泡灌洗液(BALF)中白介素(IL)-4、IL-13和血清总Ig E的水平,Western blot检测肺组织NF-κB蛋白表达。结果 :哮喘组小鼠气道炎症和气道高反应性明显加重,白杨素能够显著抑制哮喘模型小鼠的气道炎症和气道高反应性,白杨素能够抑制哮喘小鼠BALF中IL-4、IL-13和血清总Ig E水平。哮喘组小鼠肺组织高表达的NF-κB水平,经白杨素治疗后显著降低。结论:白杨素能够抑制哮喘小鼠的气道炎症和气道高反应性,其机制可能与抑制肺组织NF-κB的表达有关。
Objective: To observe the effect of chrysin on the expression of nuclear factor (NF) -κB in lung tissue of acute asthma model mice. Methods: Twenty-four female BALB / c mice were randomly divided into 4 groups: normal control group, asthma group, chrysin and budesonide groups, 6 in each group. Asthma model was induced by intraperitoneal injection of ovalbumin and airway stimulation. Chrysin was intragastrically administered to chrysin at 50 mg / kg. Budesonide group was given budesonide aerosol inhalation. Pulmonary function tests evaluated airway resistance in mice, airway inflammation in mice was assessed by HE staining, interleukin-4 (IL-4), interleukin-13 (IL-13) and total serum in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay Ig E levels, Western blot detection of NF-κB protein expression in lung tissue. Results: Airway inflammation and airway hyperresponsiveness were significantly increased in asthmatic mice. Chrysin significantly inhibited airway inflammation and airway hyperresponsiveness in asthmatic mice. Chrysin could inhibit IL-4 and IL-4 in BALF in asthmatic mice -13 and total serum IgE levels. The level of NF-κB in lung tissue of asthmatic mice significantly decreased after chrysin treatment. CONCLUSION: Chrysin can inhibit airway inflammation and airway hyperresponsiveness in asthmatic mice, which may be related to the inhibition of NF-κB expression in lung tissue.