采用PCR法克隆了桃褐腐病菌的角质酶基因cut1,构建了诱导型表达载体pET28a-cut1,转化大肠杆菌E.coli(BL21),获得重组菌pET28a-cut1/BL21。经异丙基-β-D-硫代吡喃半乳糖苷(IPTG)诱导,该重组菌角质酶的表达量约为对照菌的1.69倍,酶活约为对照菌的1.8倍,粗酶液的酶活可达40.33 U/mL。 The cutinase gene cut1 was cloned by PCR and the inducible expression vector pET28a-cut1 was constructed. The recombinant plasmid pET28a-cut1 / BL21 was transformed into E. coli (BL21). After induced by isopropyl-β-D-thiogalactopyranoside (IPTG), the expression level of this recombinant bacteriocin was about 1.69 times that of the control and the activity was about 1.8 times of that of the control. The crude enzyme solution Enzyme activity up to 40.33 U / mL.