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目的研究己酮可可碱(PTX)对于内毒素性急性肺损伤(ALI)鼠肺磷脂酶 A2(PLA_2)和表面活性物质(主要成分为磷脂酰胆碱 PC 和磷脂酰乙醇胺 PE)的影响。方法健康鼠分4组,静脉注射内毒素建立急性肺损伤的模型,应用 PTX 治疗后,检测支气管肺泡灌洗液(BALF)和肺组织内 PLA_2的活性和 PC、PE 含量。结果内毒素诱导的鼠 ALI 中,动物 BALF 和肺组织内 PLA 的活性均明显升高,PC 下降,PE 增加(P<0.05)。而 PTX 的使用均抑制了上述各变化(P<0.05),并明显改善肺组织病理变化。结论 PTX 通过抑制 ALI 鼠肺 PLA_2的活性,提升肺表面活性物质的含量,而抑制了 ALI 的发生与发展,对 ALI 有一定的治疗作用。
Objective To investigate the effects of pentoxifylline (PTX) on pulmonary surfactant phospholipase A2 (PLA2) and surfactant (phosphatidylcholine PC and phosphatidylethanolamine PE) in rats with endotoxin-induced acute lung injury (ALI). Methods Healthy rats were divided into 4 groups. The models of acute lung injury were established by intravenous injection of endotoxin. After treatment with PTX, the activity of PLA 2 and contents of PC and PE in bronchoalveolar lavage fluid (BALF) and lung tissue were measured. Results In endotoxin-induced ALI, the activities of PLA in BALF and lung tissue of animals were significantly increased, PC decreased and PE increased (P <0.05). The use of PTX inhibited the above changes (P <0.05), and significantly improved lung tissue pathology. Conclusions PTX can inhibit the occurrence and development of ALI by inhibiting the activity of PLA_2 in lung of ALI rats and increasing the content of pulmonary surfactant, which has a certain therapeutic effect on ALI.