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目的:检测宫颈癌组织中Ras相关区域家族2A(RASSF2A)基因启动子甲基化状态,并分析RASSF2A基因甲基化率与临床病理特征的关系。方法:采用甲基化特异性PCR(MSP)检测46例宫颈癌、38例宫颈上皮内瘤变(CIN)和25例正常宫颈组织中RASSF2A基因启动子甲基化状态。结果:宫颈癌组织中RASSF2A基因甲基化率显著高于CIN组织及正常宫颈组织(P<0.05)。RASSF2A甲基化阳性率随着淋巴结转移而升高,具有明显的相关性(P<0.05);不同年龄、临床分期、组织分级及病理性类型与RASSF2A基因甲基化无显著相关性(P>0.05)。结论:RASSF2A基因启动子甲基化为频发事件,可能与宫颈癌的发生和淋巴结转移有关。
Objective: To detect the promoter methylation status of RASSF2A gene in cervical cancer and to analyze the relationship between RASSF2A methylation and clinicopathological features. Methods: Methylation-specific PCR (MSP) was used to detect the methylation status of RASSF2A promoter in 46 cases of cervical cancer, 38 cases of cervical intraepithelial neoplasia (CIN) and 25 cases of normal cervical tissue. Results: The methylation rate of RASSF2A gene in cervical cancer was significantly higher than that in CIN and normal cervical tissues (P <0.05). The positive rate of methylation of RASSF2A increased with lymph node metastasis (P <0.05). There was no significant correlation between methylation of RASSF2A methylation and RASSF2A gene methylation in different age, clinical stage, histological grade and pathological type (P> 0.05). Conclusion: Methylation of RASSF2A promoter is a frequent event, which may be related to the occurrence of cervical cancer and lymph node metastasis.