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目的:研究全蝎蛋白药效组分的提取方法,摸索全蝎蛋白药效组分SDS-PAGE的最佳电泳条件,测定全蝎蛋白药效组分电泳图谱条带的大致分子质量,确定其SDS-PAGE的表达特征,从而建立全蝎蛋白药效组分的电泳鉴别法。方法:采用水提与盐析法获得全蝎蛋白药效组分,考马斯亮蓝法测定蛋白药效组分的蛋白质含量,SDS-PAGE法和AlphaEaseFC4.0.0软件测定全蝎蛋白药效组分的蛋白质相对分子质量。结果:全蝎蛋白药效组分的蛋白质质量分数为50.37%;得到较好的全蝎蛋白药效组分的10条电泳谱带,相对分子质量分别为94.1620,66.4000,40.3570,27.5410,22.5180,19.5200,12.7660,11.1900,9.6653,8.5342KDa。结论:这10条谱带是全蝎蛋白药效组分的特征谱带,可用于全蝎蛋白药效组分的鉴别。
OBJECTIVE: To study the extraction method of pharmacologically active components of scorpion proteins and explore the optimal electrophoresis conditions of SDS-PAGE of scorpion proteins. The approximate molecular mass of electrophoresis bands of scorpion proteins was determined, SDS-PAGE expression characteristics, thus establishing a scorpion protein electrophoresis method of electrophoresis. Methods: The protein fractions of the whole scorpion protein were obtained by water extraction and salting out. The protein content of the protein fractions was determined by Coomassie Brilliant Blue method. SDS-PAGE and AlphaEaseFC4.0.0 Protein relative molecular mass. Results: The protein mass fraction of the drug components of the scorpion protein was 50.37%; 10 electrophoresis bands of the drug components of the scorpion protein were obtained, with the relative molecular masses of 94.1620, 66.4000, 40.3570, 27.5410, 22.5180, 19.5200, 12.7660, 11.1900, 9.6653, 8.5342 KDa. Conclusion: These 10 bands are the characteristic bands of the scorpion proteins and can be used to identify the pharmacodynamic components of the whole scorpion proteins.