Abstract [Objective] This study was to investigate the effect of forsythiaside A added in chick embryo kidney cells （CEKs）, on the changes in expression of inter- feron c~ （IFN-cQ and mRNA expression level of correlated factors in JAK-STAT path- way. [Methods] Three levels of forsythiaside （100, 200, 400 ~g/ml） were adopted to treat the experimental materials, then the expression levels of IFN-cc and correlated factors in JAK-STAT pathway were detected by real-time RT-PCR at the transcription- al level （mRNA） and by Western blot at the translation level （IFN-α~ protein）. [Result] Compared with the control group （untreated group）, Application of forsythiaside A not only significantly increased the expression of IFN-α in treated CEKs （P〈0.05） , but also up-regulated the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1 and IRF7, the correlated factors in JAK-STAT pathway. [Conclusion] Forsythiaside A in- duced the expression of IFN-cc in CEKs, and can positively regulate the JAK-STAT signaling pathway significantly.