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根据菘蓝转录组数据,克隆了菘蓝莽草酸羟基肉桂酸酰转移酶Ii HCT的全长c DNA并进行生物信息学分析,进一步利用实时荧光定量PCR技术分析了Ii HCT基因的表达模式。Ii HCT ORF为1 290 bp,编码430个氨基酸,等电点为5.77,相对分子质量为47.68 k Da。Ii HCT主要在菘蓝茎中表达,幼根、叶、花蕾中几乎不表达。同时发现离体菘蓝毛状根中可以检测到Ii HCT的表达,外源茉莉酸甲酯(Me JA)处理后,Ii HCT相对表达量明显增加,在4 h达到原先的4.3倍。研究首次从菘蓝中克隆得到HCT基因,为进一步解析菘蓝苯丙素类成分的生物合成途径奠定基础。
According to the transcriptome data of B. tuberosus, the full-length c DNA of Ih HCT was cloned and analyzed by bioinformatics analysis. The expression pattern of I HCT was further analyzed by real-time fluorescence quantitative PCR. The I HCT ORF was 1 290 bp, encoding 430 amino acids with an isoelectric point of 5.77 and a relative molecular mass of 47.68 kDa. Ii HCT is mainly expressed in Scylla sylvestris, almost not expressed in young roots, leaves and buds. At the same time, the expression of Ii HCT was detected in the hairy roots of the Prunus mume. The expression of Ii HCT increased significantly after treated with Me JA, reaching 4.3 times of that at 4 h. HCT gene was cloned for the first time from Isatis indigotica so as to lay the foundation for the further analysis of the biosynthesis pathway of 菘 phenylpropanoid components.