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目的:研究宫颈鳞状上皮病变中细胞凋亡的情况及其意义。方法:选择2008年1月～2009年10月桂林医学院附属医院53例宫颈组织制成组织芯片,其中正常9例,CIN 32例,鳞状细胞癌12例;应用TUNEL法检测细胞凋亡情况。结果:凋亡阳性率依次为:正常组0%(0/9),CIN组15.62%(5/32),鳞状细胞癌66.67%(8/12);正常组、CIN组与癌症组凋亡阳性率比较差异有统计学意义(P<0.01)。结论:细胞凋亡现象随着宫颈鳞状上皮病变的发展逐渐明显,应用TUNEL法检测宫颈组织芯片,具有优质、高效、结果可靠的特点。
Objective: To study the apoptosis of cervical squamous cell carcinoma and its significance. Methods: From January 2008 to October 2009, 53 cases of cervical tissue from the Affiliated Hospital of Guilin Medical College were made into tissue microarrays, including 9 cases of normal, 32 cases of CIN and 12 cases of squamous cell carcinoma. TUNEL method was used to detect the cell apoptosis . Results: The positive rate of apoptosis was 0% (0/9) in normal group, 15.62% (5/32) in CIN group and 66.67% (8/12) in squamous cell carcinoma. In normal group and CIN group, The positive rate of death was statistically significant (P <0.01). Conclusion: With the development of cervical squamous cell lesion, the phenomenon of apoptosis is gradually obvious. TUNEL method is used to detect cervical tissue microarray. It has the characteristics of high quality, high efficiency and reliable results.