本文叙述了一个用高压液相色谱测定鱼肝脏中维生素E的简便方法。此方法不需要皂化和薄层层析,这样就避免了在样品处理过程中α-生育酚受到破坏。从3～4条鱼的肝脏中每个取样约0.5 g,称重。样品在15 ml二(口恶)烷-异辛烷(20+80)混合液中匀浆1分钟。匀浆在每分钟10,000转的转速下离心5分钟。吸5 ml上清液于250 ml园底烧瓶中。弃去剩余上清液,沉淀物再用15 ml混合液匀浆,离心,取5 ml This article describes a simple and convenient method for determination of vitamin E in fish liver by high pressure liquid chromatography. This method does not require saponification and thin layer chromatography, thus avoiding α-tocopherol destruction during sample processing. Approximately 0.5 g was taken from each of 3 to 4 fish livers and weighed. Samples were homogenized in 15 ml of dioxane-isooctane (20 + 80) for 1 minute. The homogenate is centrifuged at 10,000 rpm for 5 minutes. Suction 5 ml supernatant in 250 ml round bottom flask. The remaining supernatant was discarded and the pellet was homogenized with 15 ml of the mixture and centrifuged for 5 ml