反意RNA(一种调控RNA)能与特异基因的mRNA互补,形成双螺旋,并抑制该基因的表达,从而成为调节基因表达的一个有效工具。与用同源性基因重排原理调节基因表达的方法不同,此法的抑制效果来自基因关闭而非基因交换。过去曾报道的反意RNA调节植物基因的实验是应用了暂时表达体系,将顺意和反意基因与启动子结合后,再转化至植物胞浆,该基因由于反意RNA作用而不被表达。在已转化有外源基因的植物系中,又进一步转化相应的反意基因后,发现原来能稳定表达的外源基因不再表达了。在这些实验 Anti-sense RNA, a regulatory RNA, complements the mRNA of a specific gene to form a double helix and inhibits its expression, making it an effective tool for regulating gene expression. Unlike the method of regulating gene expression using the principle of homologous gene rearrangement, the inhibitory effect of this method comes from gene shut down instead of gene exchange. In the past, it was reported that the regulation of plant genes by anti-sense RNAs was based on the application of a transient expression system. After the sense and anti-sense genes were combined with the promoter and then transformed into the plant cytoplasm, the gene was not expressed due to the anti-sense RNA effect. In the plant lines transformed with exogenous genes, after further transformation of the corresponding anti-sense genes, it was found that the foreign genes originally stably expressed are no longer expressed. In these experiments