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[Objective] The paper was to compare different extraction methods for genomic DNA of fall webworm [Hyphantria cunea( Drury) ]and study the amplification effect of different primers on sequences of CO I and CO II and Cytb.[Method] With winter pupae of fall webworm as the test materials,genomic DNA of fall webworm were extracted by five different methods including CTAB method,SDS method,improved SDS method,kit method and different processing time.Six groups of different primers were used to amplify sequences of CO I and CO II and Cytb.[Result] Full DNA could be extracted by CTAB method,SDS method and improved SDS method,and the effort of low concentration SDS method was the best.Six groups of primers could amplify the corresponding fragment,and two groups of primers had the best effort for amplification of CO I.[Conclusion] The study provides better methods for DNA extraction.
[Objective] The paper was to compare different extraction methods for genomic DNA of fall webworm [Hyphantria cunea (Drury)] and study the amplification effect of different primers on sequences of CO I and CO II and Cytb. [Method] With winter pupae of fall webworm as the test materials, genomic DNA of fall webworm were extracted by five different methods including CTAB method, SDS method, improved SDS method, kit method and different processing time. Mix groups of different primers were used to amplify sequences of CO I and CO II and Cytb. [Result] Full DNA could be extracted by CTAB method, SDS method and improved SDS method, and the effort of low concentration SDS method was the best. Mix groups could not amplify the corresponding fragment, and two groups of primers had the best effort for amplification of CO I. Conclusion The study provides better methods for DNA extraction.