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目的获得B病毒gC蛋白的特异性表位抗原。方法利用长片段基因合成的方法,合成B病毒C蛋白的特性抗原表位基因,将该基因连接到pMAL-5x载体,转化到BL21受体菌进行表达,并纯化表达产物。结果成功的获得了B病毒gC蛋白的特异性抗原蛋白,该蛋白以可溶的形式表达。结论利用原核表达系统,可以产生B病毒gC蛋白的可溶性抗原,可以作为B病毒的检测抗原。
Objective To obtain the specific epitope antigen of B virus gC protein. Methods Using the method of long fragment gene synthesis, the antigenic epitope gene of protein B of B virus was synthesized. The gene was ligated into pMAL-5x vector and transformed into BL21 receptor. The expressed product was purified. As a result, the specific antigenic protein of B virus gC protein was successfully obtained, and the protein was expressed in a soluble form. Conclusion The prokaryotic expression system can produce the soluble antigen of B virus gC protein and can be used as the detection antigen of B virus.