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目的：了解侵袭性骨肿瘤增殖、凋亡与其生物学行为的关系。方法：用免疫组织化学方法对骨肉瘤、软骨肉瘤、骨巨细胞瘤石蜡包埋组织内ｃ－ｍｙｃ、Ｂｃｌ－２癌基因蛋白表达水平、增殖细胞核抗原和凋亡小体数（ＨＥ染色）进行检测。结果：ｃ－ｍｙｃ、Ｂｃｌ－２蛋白表达水平及凋亡小体数均以骨肉瘤为最高，软骨肉瘤次之，骨巨细胞瘤最低；增殖细胞核抗原标记指数亦以骨肉瘤为最高，但骨巨细胞瘤次之，软骨肉瘤最低。结论：凋亡相关指标与３种骨肿瘤生物学行为关系密切，而增殖细胞核抗原标记指数虽能客观反映肿瘤的增殖程度，但与它们各自的生物学行为关系不密切。
Objective: To understand the relationship between proliferation, apoptosis and biological behavior of invasive bone tumors. Methods: The expression of c-myc and Bcl-2 oncogene proteins, proliferating cell nuclear antigen and number of apoptotic bodies (HE staining) in paraffin-embedded tissues of osteosarcoma, chondrosarcoma, and giant cell tumor of bone were examined by immunohistochemistry. Testing. RESULTS: The expression of c-myc and Bcl-2 protein and the number of apoptotic bodies were highest in osteosarcoma, followed by chondrosarcoma, and giant cell tumor of bone. Proliferative cell nuclear antigen marker was also highest in osteosarcoma but bone Giant cell tumors followed, and cartilage sarcoma had the lowest. Conclusion: Apoptosis-related markers are closely related to the biological behavior of three kinds of bone tumors. Proliferating cell nuclear antigen marker index can objectively reflect the degree of tumor proliferation, but is not closely related to their respective biological behaviors.