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受检血清、抗原及抗血清的制备:在班氏丝虫病流行区及非流行区分别从当地人群采血529份和50份,离心获取血清,于-20℃保存待用。棉鼠丝虫成虫取自大鼠胸腔,PBS洗净,匀浆超声粉碎,离心(20,000g、4℃、20分钟)并测取280～260nm吸收光波段中的蛋白含量,调整至15mg/ml即为实验用可溶性抗原。并提取班氏微丝蚴、圆线虫及蛔虫抗原。用上述棉鼠丝虫可溶性抗原0.75ml混合等量弗氏完全佐剂隔周皮下免疫家兔,于第3次免疫后10天放血、离心,取得兔抗棉鼠丝虫血清。
Serum, antigen and antiserum were collected: 529 and 50 samples of blood were collected from endemic areas and endemic to Bancroftian’s filariasis respectively. The serum was collected by centrifugation and stored at -20 ℃ for later use. The adult mice were taken from the rat’s thoracic cavity, washed with PBS, homogenized and pulverized by ultrasound, centrifuged (20,000 g, 4 ° C, 20 min) and the protein content in the absorption band of 280-260 nm was measured to adjust to 15 mg / ml Is the experimental use of soluble antigen. And extract Bancrofeex microflora, round worm and roundworm antigen. Rabbits were immunized subcutaneously with the same amount of Freund’s complete adjuvant 0.75 ml mixed with the fibroblast-soluble antigen of the above-mentioned cotton fibroids for 10 days after the third immunization, and then centrifuged to obtain the rabbit anti-cotton silkworm serum.