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目的检测初发和复发尖锐湿疣(CA)患者皮损及外周血T细胞亚群中TLR9的表达情况,并探讨TLR9在HPV感染和CA复发机制中的作用。方法分别选用30例初发CA和27例复发CA患者皮损,并以28例正常人皮肤石蜡切片作为正常对照组。采用双色免疫荧光抗体染色流式细胞术检测入选者外周血T细胞中TLR9的表达。结果初发CA组和复发CA组皮损颗粒层和棘层TLR9表达(1.38±0.72和2.06±0.84)和外周血CD3+CD4+T细胞内TLR9表达[(11.4±3.2)%和(14.6±2.4)%]显著高于正常对照组[0.97±0.43和(6.2±2.1)%];另外,复发CA患者外周血CD3+CD4+T细胞内TLR9的表达也显著高于初发CA组;以上差异均有统计学意义(P均<0.05)。但是该两组患者的外周血CD3+CD8+T细胞内TLR9的表达与正常对照组差异不显著(P>0.05)。结论 CA皮损及外周血CD3+CD4+T细胞内TLR9表达上调可能是HPV病毒感染的识别受体,并参与机体的抗HPV免疫应答。TLR9可能在CA复发机制中发挥着重要的作用。
Objective To detect the expression of TLR9 in the skin lesions and peripheral blood T lymphocyte subsets in patients with primary and recurrent condyloma acuminatum (CA) and to explore the role of TLR9 in HPV infection and CA recurrence. Methods The lesions of 30 patients with primary CA and 27 patients with recurrent CA were selected, and 28 normal skin paraffin sections were used as normal control group. Two-color immunofluorescence staining was used to detect the expression of TLR9 in the peripheral blood T cells of the recipients. Results The expressions of TLR9 (1.38 ± 0.72 and 2.06 ± 0.84) and TLR9 in peripheral blood CD3 + CD4 + T cells (11.4 ± 3.2%) and (14.6 ± 2.4)%] was significantly higher than that of the normal control group [0.97 ± 0.43 and (6.2 ± 2.1)%]. In addition, the expression of TLR9 in peripheral blood CD3 + CD4 + The differences were statistically significant (all P <0.05). However, the expression of TLR9 in peripheral blood CD3 + CD8 + T cells in these two groups was not significantly different from the normal control group (P> 0.05). Conclusion The up-regulation of TLR9 expression in CA lesions and CD3 + CD4 + T cells in peripheral blood may be the recognition receptor for HPV infection and participate in the anti-HPV immune response in the body. TLR9 may play an important role in the mechanism of CA recurrence.