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[目的 ]评价以恰氏利氏曼原虫类 kinesin基因中编码 39个氨基酸的基因片段 (r K39)为重组抗原 ,用于血清学诊断内脏利什曼病的价值。 [方法 ]在新疆喀什地区对 13例经脾检和骨髓穿刺阳性的内脏利什曼病患者 ,取一滴病人全血或血清滴在 r K39抗原试纸条底部的吸收垫上 ,血清中蛋白随缓冲液向试纸条上部移动 ,其中相应特异抗体可与 r K39抗原带结合 ,而产生阳性条带。同时 ,本文亦用相同阳性血清作了关于 r K 39抗原的 Western印迹分析对照。 [结果 ]EL ISA分析显示病人血清抗体滴度在 10 - 2～ 10 - 4 ,与所见到的 r K39试纸条上的反应强度符合。Western印迹分析亦显示阳性血清可识别 r K39蛋白条带。[结论 ]与传统诊断内脏利什曼病方法相比较 ,r K39试纸条更快速 ,特异 ,灵敏和低损伤性 ,可用于低发病率流行区的内脏利什曼病的诊断和筛选
[Objective] To evaluate the value of using a 39-amino acid gene fragment (r K39) encoding the kinesin gene of Chagasi as a recombinant antigen for serological diagnosis of visceral leishmaniasis. [Method] Thirteen cases of visceral leishmaniasis in spleen and bone marrow aspirates were taken from Kashgar region in Xinjiang. One drop of patient’s whole blood or serum was dropped on the absorbent pad at the bottom of r K39 antigen test strip. The protein in the serum with buffer The liquid moves to the upper part of the test strip where the corresponding specific antibody binds to the rK39 antigen band and a positive band is produced. In the meantime, the same positive sera was also used as a control for Western blot analysis of the rK39 antigen. [Results] EL ISA analysis showed that the titer of the patient ’s serum antibody was between 10 - 2 and 10 - 4, which was in accordance with the reaction intensity of the r K39 test strip. Western blot analysis also showed that positive sera recognized the rK39 protein band. [Conclusion] Compared with the traditional method of diagnosing visceral leishmaniasis, r K39 test strip is more rapid, specific, sensitive and less damaging and can be used in the diagnosis and screening of visceral leishmaniasis in endemic areas with low prevalence