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目的 :研究乙型肝炎病毒 (HBV)核心启动子 (BCP)区 176 2位和 176 4位热点突变对HBeAg存在状态的影响。方法 :采用错配引物的PCR结合限制性内切酶技术检测 90例不同HBeAg状态的HBV无症状携带者的HBVBCP热点突变。结果 :6 0例HBeAg阴性者中HBVBCP热点突变者 2 6例 (4 3.3% ) ,其中 2 0例伴有HBV前C区 (Pre C)区1896位热点突变 ;30例HBeAg阳性者中仅 3例 (10 % )出现HBVBCP热点突变 ;无HBVPre C区热点突变者中 ,19例HBeAg阴性者有 6例存在HBVBCP热点突变 (31.6 % ) ,2 8例HBeAg阳性者仅 2例有HBVBCP热点突变 (7.1% ) ,两组间比较差异具显著性 (P <0 .0 5 )。结论 :HBVBCP热点突变在HBeAg阴性HBV感染者中较常见 ,且常伴有HBVPre C区 1896位突变 ,HBVBCP热点突变可能是HBeAg阴性的另一原因
Objective: To study the effect of hot spots mutation at 176 2 and 176 4 of hepatitis B virus (HBV) core promoter (BCP) on HBeAg status. Methods: HBV hotspot mutation in HBV asymptomatic carriers of 90 HBeAg-positive HBV carriers was detected by PCR and restriction endonuclease analysis. Results: Of the 60 HBeAg-negative patients, 26 were HBV hot spot mutations (4.3%), 20 of which were associated with 1896 hot spot mutations in pre-C precancerous lesions and only 3 of 30 HBeAg-positive patients (10%) showed hot spot mutation of HBV BCP. Of the 19 HBeAg-negative patients without HBV Pre C mutation, 6 cases had HBVBCP hot spot mutation (31.6%), and 2 of 28 HBeAg positive patients had HBV BCP hot spot mutation 7.1%), the difference between the two groups was significant (P <0.05). Conclusion: The hot spot mutation of HBV BCP is more common in HBeAg-negative patients with HBV infection, and is often accompanied by a 1896 mutation in HBV Pre C region. The mutation of HBV BCP hot spot may be another reason of negative HBeAg