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目的 比较不同低强度微波辐射对体外培养兔眼晶状体上皮细胞增殖活性及细胞周期的影响 ,初步探讨晶状体上皮细胞对微波辐射损伤的耐受剂量。方法 在兔晶状体上皮细胞体外培养的基础上 ,应用频率 2 4 5 0MHz,功率密度分别为 0 .10、0 .2 5、0 .5 0、1.0 0、2 .0 0mW cm2 的微波连续辐射细胞 8h ,HE染色 ,观察辐射前后细胞形态学改变 ,四唑盐 (MTT)比色法测定细胞增殖活性 ,并用PI荧光染色检测微波辐射对细胞周期时相的影响。结果 0 .5 0、1.0 0、2 .0 0mW cm2 微波组辐射晶状体上皮细胞 8h后 ,均可降低细胞增殖活性 ,使细胞排列紊乱、固缩或脱壁 ,同时抑制细胞DNA合成 ,G0 G1 期细胞百分比分别为 71.95 %± 2 .12 %、75 .6 8%± 3.35 %、82 .4 0 %± 8.6 8% ,高于对照组( 6 1.6 8%± 5 .76 % ) ,差异有显著性 (P <0 .0 5或P <0 .0 1) ,而S期细胞所占比例分别为 19.32 %±3.0 7%、16 .0 8%± 4 .91%、12 .98%± 8.0 8% ,较对照组 ( 2 8.0 5 %± 5 .12 % )明显减少 ,差异有显著性 (P<0 .0 5或P <0 .0 1)。功率密度为 0 .10、0 .2 5mW cm2 的微波组对细胞增殖活性和细胞周期未见影响 ,与对照组的差异无显著性 (P >0 .0 5 )。结论 功率密度高于 0 .5 0mW cm2 的微波连续辐射 8h ,可对体外培养的兔晶状?
Objective To compare the effect of different low-intensity microwave radiation on the proliferation and cell cycle of cultured rabbit lens epithelial cells in vitro and to explore the tolerance dose of lens epithelial cells to microwave irradiation. Methods Based on the cultured rabbit lens epithelial cells cultured in vitro, the cells were irradiated continuously with microwave at a frequency of 2450 MHz and power densities of 0.010, 0.52, 5.0, 5.0, 2.0, 8h, HE staining, morphological changes before and after irradiation, cell proliferation activity was measured by MTT colorimetric assay, and the effect of microwave irradiation on the cell cycle phase was detected by PI fluorescence staining. Results After irradiation of lens epithelial cells at 0 .5 0,1.0 0,2. 0 0mW cm2 for 8h, the cell proliferation activity was decreased and the cells were disordered, condensed or detached, and the DNA synthesis was inhibited. The G0G1 phase The percentages of cells were 71.95% ± 2.12%, 75.68% ± 3.35%, 82.4% ± 8.68%, respectively, which were significantly higher than those in the control group (6 1.6 8% ± 5.76%) (P <0.05 or P <0.01), while the percentage of S phase cells were 19.32% ± 3.0 7%, 16.0 8% ± 4.91%, 12.98% ± 8.0 8%, which was significantly lower than that of the control group (28.05% ± 5.12%) (P <0.05 or P <0.01). There was no significant difference in cell proliferation activity and cell cycle between microwave group with power density of 0 .10,0. 25mW cm2 and control group (P> 0.05). Conclusion The continuous microwave irradiation with a power density higher than 0.55 mW cm2 for 8 h,