利用离子对色谱-电化学检测器建立一种高灵敏、快速的检测方法,用于脑组织中硫醇和鸟苷代谢物的同时测定.采用Agilent SB-AQ色谱柱,以100 mmol/L V(磷酸缓冲盐,pH 2.0,含350 mg/L辛烷基磺酸钠):V(甲醇)=95:5为流动相进行分离,检测电压为800 mV.所测的6种硫醇和鸟嘌呤类物质在15 min内得到完全分离,且在测定浓度范围内线性良好.检出限为22.6~56.9 nmol/L,不同加标水平的回收率为94.4%~106.3%,变异系数为0.21%~3.85%.该方法可用于脑组织中硫醇和鸟嘌呤类物质的同时测定. A sensitive and rapid detection method for the simultaneous determination of thiols and guanosine metabolites in brain tissue was established by ion-pair chromatography-electrochemical detector.Using an Agilent SB-AQ column, Buffer salt, pH 2.0, containing 350 mg / L sodium octane sulfonate): V (methanol) = 95: 5 as the mobile phase at a detection voltage of 800 mV The measured six thiol and guanine species The separation was complete within 15 min with a good linearity within the concentration range.The detection limit was 22.6-56.9 nmol / L, the recoveries of different spiked levels were 94.4% -106.3%, and the coefficients of variation were 0.21% -3.85% This method can be used for the simultaneous determination of thiols and guanines in brain tissue.