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以甜瓜连作土壤为研究对象,采用高效液相色谱技术,分析了不同连作年限甜瓜土壤中酚酸物质的种类及其含量变化,利用生防木霉菌降解特性,降解土壤中酚酸类物质,为缓解甜瓜连作障碍提供参考依据。结果表明:连作甜瓜土壤中对羟基苯甲酸、香草酸、丁香酸、香兰素、香豆酸、阿魏酸、苯甲酸随着连作年限的增加而增加,连作3年后7种酚酸的总量达245.39mg·kg~(-1)干土,显著高于未连作土壤;采用孢子菌悬液、PD菌悬液和麦麸发酵物3种不同的接种方式,测定木霉菌T23对甜瓜根际土壤中酚酸类物质的降解作用,表明木霉菌T23能够有效降解土壤中积累的酚酸物质,其中麦麸发酵物降解率可达到25%以上,降解效果最为显著。
Taking melon continuous cropping soil as research object, using high performance liquid chromatography (HPLC), the changes of phenolic acids and their contents in melon soil in different continuous cropping years were analyzed. The degrading properties of Trichoderma antifungal were used to degrade phenolic acids in soil Ease melon continuous cropping obstacles provide a reference. The results showed that the contents of p-hydroxybenzoic acid, vanillic acid, syringic acid, vanillin, coumaric acid, ferulic acid and benzoic acid increased with the continuous working years, Total amount of 245.39 mg · kg -1 dry soil was significantly higher than that of the soil without continuous cropping. Three different inoculation methods of spore suspension, PD suspension and wheat bran fermentation were used to determine the effect of Trichoderma T23 on the growth of melon The degradation of phenolic acids in rhizosphere soil indicated that Trichoderma T23 could effectively degrade the phenolic acids accumulated in the soil. The degradation rate of wheat bran ferment could reach over 25% and the degradation effect was the most significant.