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目的:建立定风止痛片中乌头碱含量测定方法。方法:采用色谱柱:Kromasil 100-5C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-0.1%氨水(磷酸调pH至9.0)(40∶60);检测波长为240nm,柱温:30℃,流速:1.0mL.min-1。结果:乌头碱在0.0249～0.249μg/mL范围内呈良好的线性关系,回归方程为:Y=256.02X+1.3257(r=0.9998),加样回收率为99.60%(RSD=2.70%,n=6)。结论:该法操作简便,结果准确,可以用作测定定风止痛片中乌头碱含量,为定风止痛片的质量控制方法提供参考。
Objective: To establish a method for determination of aconitine in Dingfeng Zhitong Tablet. Methods: Kromasil 100-5C18 column (250 mm × 4.6 mm, 5 μm) was used as the mobile phase. The mobile phase was acetonitrile-0.1% aqueous ammonia (adjusted to pH 9.0 with phosphoric acid) (40:60). The detection wavelength was 240 nm, 30 ° C, flow rate: 1.0 mL.min-1. Results: Aconitine had a good linearity in the range of 0.0249-0.249μg / mL. The regression equation was: Y = 256.02X + 1.3257 (r = 0.9998), and the recoveries were 99.60% (RSD = 2.70%, n = 6). Conclusion: The method is simple and accurate. It can be used to determine the content of aconitine in Dingfeng Zhitong Tablet and provide a reference for quality control of Dingfeng Zhitong Tablet.