一、定量分析根据性甾体激素放射免疫测定法,将啥士蟆油研成粉末,用乙醚在涡旋器上提取脂溶性成分。将脂溶性成分用苯一甲醇(85∶15)混合液溶解,加到Sephadex LH_2O柱上,根据~3H雌二醇、~3H孕酮和(?)H睾酮在凝胶柱上所流下的位置,分别收集雌二醇、孕酮、睾酮流分、蒸发、干燥、测定含量(pg/100mg):睾酮15.3±1.4,孕酮187.9±19.4,雌二醇52.3±5.89。二、药理实验将哈士蟆油脂溶性成分加生理盐水和吐温80适量,制成注射液。浓度 I. Quantitative analysis According to the steroid hormone radioimmunoassay method, the gentian oil was ground into a powder, and the fat-soluble component was extracted with ether on a vortexer. The fat-soluble components were dissolved in a mixture of benzene-methanol (85:15) and applied to a Sephadex LH 2 O column according to the position of ~3H estradiol, ~3H progesterone and (?)H testosterone on the gel column. The estradiol, progesterone, testosterone fractions, evaporation, drying, and assay levels (pg/100 mg) were collected: testosterone 15.3±1.4, progesterone 187.9±19.4, estradiol 52.3±5.89. Second, pharmacological experiments Halsea oil soluble ingredients plus normal saline and Tween 80 amount, made of injection. concentration