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目的比较不同年龄小鼠体外受精胚胎发育的结局,探讨以年龄因素为背景的IVF胚胎发育的影响。方法采用相同超数排卵方案,在相同的培养条件和培养时间下,对4-8周龄和44-48周龄小鼠进行体外受精和胚胎培养,观察并记录双原核卵率、≥4细胞率、囊胚率以及孵化囊胚率。结果老龄组在受精率、卵裂率以及孵化囊胚率上与年轻组比较存在极显著性差异(P<0.01),但在囊胚形成率上的差异无统计学意义;老龄鼠超数排卵后获卵数明显少于年轻小鼠。结论年龄对卵子数量以及IVF胚胎早期发育有较大影响。
Objective To compare the embryo development of in vitro fertilized mice of different ages and to explore the influence of IVF embryo development on the background of age factors. Methods The same superovulation scheme was used to in vitro fertilization and embryo culture of 4-8 weeks old and 44-48 weeks old mice under the same culture conditions and culture time. Rate, blastocyst rate and hatched blastocyst rate. Results There was a significant difference in fertilization rate, cleavage rate and hatched blastocyst rate between the aging group and the young group (P <0.01), but there was no significant difference in the blastocyst formation rate between the aging group and the young group Obviously less eggs than young mice. Conclusion Age has a great effect on the number of eggs and the early development of IVF embryos.