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目的:制备新型挫伤型脊髓损伤大鼠模型,通过行为学评分和形态学方法进行评价,为进一步研究脊髓损伤的机制及早期治疗提供依据。方法:SD大鼠随机分为对照组和实验组,对照组(A组)采用改良的Allen法制备急性脊髓挫伤模型,实验组在打击的基础上分别受压3s(B组)、5s(C组)和10s(D组),制备新型挫伤型脊髓损伤模型;各组分别于术后1、3、7、14、21d观察其行为学评分和病理学改变;免疫组织化学方法检测促炎因子高迁移率族蛋白B1(HMGB1)在各组不同时间点的表达变化。结果:实验组各时间点BBB评分和损伤程度与对照组比较均有显著差异(P<0.05),其中A组和B组术后损伤程度差别微小;C组后肢运动功能障碍明显,脊髓损伤区域出现典型的病理改变;D组损伤最严重,死亡率高。HMGB1表达以损伤C组为典型代表,术后阳性表达明显增多,第3d达高峰,主要表达部位为神经元胞浆和神经胶质细胞核内。结论:采用改良Allen法打击再受压5s制备的脊髓损伤模型能很好地模拟临床实际,且稳定性高、可复制性好,为一种新型挫伤型脊髓损伤模型。
OBJECTIVE: To prepare a new rat model of contusion-induced spinal cord injury and to evaluate it by behavioral scoring and morphological methods to provide a basis for further study on the mechanism and early treatment of spinal cord injury. Methods: Sprague-Dawley rats were randomly divided into control group and experimental group. The control group (group A) was given acute Allen contusion to establish acute spinal cord contusion model. The rats in experimental group were subjected to 3s (group B), 5s Group) and 10s (group D), a new model of contusion-induced spinal cord injury was established. The behavioral scores and pathological changes were observed at 1, 3, 7, 14 and 21 days after operation in each group. The expression of pro-inflammatory cytokines The expression of high mobility group box 1 (HMGB1) in each group at different time points changed. Results: The score of BBB and the degree of injury at each time point in the experimental group were significantly different from those in the control group (P <0.05), but there was no difference in the degree of postoperative injury between the two groups. The motor dysfunction of hindlimb in group C was obvious, A typical pathological changes appear; D group the most serious injury, high mortality. The expression of HMGB1 in injured group C was typical, and the positive expression of HMGB1 was significantly increased after operation. The expression of HMGB1 reached the peak on the third day, and was mainly located in the nucleus of neuronal cytoplasm and glial cells. Conclusion: Spinal cord injury model prepared by modified Allen method for repressurization for 5 seconds can simulate the clinical practice well and has high stability and reproducibility. It is a new model of contusion spinal cord injury.