论文部分内容阅读
目的探讨肠安Ⅰ号及含有低浓度肠安Ⅰ号成分的安慰剂对腹泻型肠易激综合征大鼠血清细胞因子TNF-α、IL-10的影响。方法大鼠随机分为正常组,模型组,含5%、含10%肠安Ⅰ号组,肠安Ⅰ号高、中、低剂量组,得舒特组共8组,每组10只。采用番泻叶灌胃+传统束缚应激刺激复制腹泻型肠易激综合征大鼠模型。各组给予相应的干预措施。治疗结束后,测定大鼠的腹泻指数和血清TNF-α、IL-10的含量。结果与正常组比较,模型组各指标差异有统计学意义(P<0.05,P<0.01),说明造模成功;与模型组比较,肠安Ⅰ号各剂量组血清中TNF-α、IL-10水平、腹泻指数差异有统计学意义(P<0.05,P<0.01);与模型组比较,含5%、含10%肠安Ⅰ号组各指标差异无统计学意义(P>0.05)。结论肠安Ⅰ号高、中、低剂量可通过增加IL-10的表达,降低TNF-α的含量,调节促炎因子抗炎因子水平,使其抗炎活性增强,从而发挥对ⅠBS的治疗作用;而含5%、含10%肠安Ⅰ号加辅料组没有药理作用,仅从中药的色、味方面模拟了肠安Ⅰ号的特点,起到了安慰剂对照作用。
Objective To investigate the effects of Geng An Ⅰ and placebo containing low-dose Changan Ⅰ on serum cytokines TNF-α and IL-10 in rats with diarrhea-predominant irritable bowel syndrome. Methods The rats were randomly divided into normal group, model group, containing 5%, containing 10% Changan Ⅰ group, Chang’an Ⅰ high, medium and low dose groups, were Shute group a total of 8 groups of 10 rats. The rat model of diarrhea-predominant irritable bowel syndrome (IBS) was stimulated by senna infusion and traditional restraint stress. Each group was given the corresponding interventions. After treatment, diarrhea index and serum TNF-α and IL-10 levels in rats were determined. Results Compared with the normal group, there were significant differences in the indexes of the model group (P <0.05, P <0.01), indicating that the model was successful. Compared with the model group, the levels of TNF-α and IL- (P <0.05, P <0.01). Compared with the model group, there was no significant difference in each index between 5% and 10% of the control group (P> 0.05). Conclusion The high, medium and low dose of Chang’an Ⅰ can increase the expression of IL-10, decrease the level of TNF-α, regulate the level of proinflammatory cytokines and anti-inflammatory cytokines and enhance its anti-inflammatory activity, Treatment with 5%, including 10% of intestinal security plus no excipients group no pharmacological effects, only from the traditional Chinese medicine color, taste simulate the safety features of No. 1, played a placebo-controlled effect.