文章将IL 7与IL 2 ,PHA及αCD3联用诱生PHA αCD3LAK细胞 ,应用活细胞计数、MTT和APAAP等方法测定了细胞增殖活性、mIL 2R的表达、外源性IL 2的消耗、细胞毒活性和效应细胞表型等指标 ,并分析比较了IL 7的加入对以上指标的影响。结果表明IL 7的加入使PHA αCD3LAK细胞消耗IL 2的量、mIL 2R表达的量和细胞的增殖能力降低 ,细胞毒活性减弱 ,并伴有CD8+ 细胞百分率的下降。这一结果呈现出的IL 7的抑制作用尚未见报道 ,有进一步研究的价值。 In this study, PHA αCD3LAK cells were induced by IL 7, IL-7, PHA and αCD3. Cell viability, expression of mIL 2R, exogenous IL 2 depletion and cytotoxicity were measured by viable cell count, MTT and APAAP assays Activity and effector cell phenotype and other indicators, and compared the impact of IL 7 on the above indicators. The results showed that the addition of IL 7 caused the amount of IL 2 to be consumed by PHA αCD3LAK cells. The amount of mIL 2R expression and the proliferation ability of the cells were decreased, and the cytotoxic activity was weakened with the decrease of the percentage of CD8 + cells. The results showed that the inhibitory effect of IL 7 has not been reported, and has further research value.