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目的探讨饮食诱导肥胖(DIO)大鼠与饮食诱导肥胖抵抗(DR)大鼠小肠糖类吸收功能的差异。方法高脂饲料建立饮食诱导肥胖与肥胖抵抗大鼠模型,8周后比较2组大鼠体重、摄食量和食物效能;随机选取8只大鼠分别进行口服葡萄糖耐量试验和口服D-木糖耐量试验,比较各时间点血糖浓度和血清D-木糖浓度。结果 DIO大鼠摄食量(417.8±33.5)kJ/d和食物效能(1.5±0.1)×100 g/kJ均高于DR大鼠(379.3±40.2)kJ/d、(1.3±0.1)×100 g/kJ,差异均有统计学意义(均P<0.05);口服葡萄糖耐量试验中DIO组曲线下面积[(16.5±1.2)mmol/(L.h)]>DR组[(14.6±1.5)mmol/(L.h)],差异有统计学意义(t=-2.75,P<0.05),口服D-木糖耐量试验2组大鼠曲线下面积差异无统计学意义。结论高脂饮食诱导的肥胖与肥胖抵抗大鼠对葡萄糖的吸收存在差异,这可能在肥胖形成中起到一定作用。
Objective To investigate the difference of intestinal carbohydrate absorption between diet-induced obesity (DIO) rats and diet-induced obesity-resistant (DR) rats. Methods Fat-induced obesity and obesity-resistant rat models were established by high-fat diet. After 8 weeks, body weight, food intake and food performance were compared between two groups. Eight rats were randomly selected for oral glucose tolerance test and oral D-xylose tolerance Test, comparing the time point blood glucose concentration and serum D-xylose concentration. Results The food intake (417.8 ± 33.5) kJ / d and food efficacy (1.5 ± 0.1) × 100 g / kJ in DIO rats were significantly higher than those in DR rats (379.3 ± 40.2 kJ / d, 1.3 ± 0.1 × 100 g / kJ (all P <0.05). In the oral glucose tolerance test, the area under the curve of DIO group [(16.5 ± 1.2) mmol / (Lh)]> DR group was significantly higher than that in DR group [(14.6 ± 1.5) mmol / Lh)], the difference was statistically significant (t = -2.75, P <0.05). There was no significant difference in the area under the curve of oral D-xylose tolerance test between the two groups. Conclusion There is a difference in glucose uptake between obese and obesity-resistant rats induced by high-fat diet, which may play a role in the formation of obesity.